The Monofunctional Catalase KatE of Xanthomonas axonopodis pv. citri Is Required for Full Virulence in Citrus Plants
Identifieur interne : 001991 ( Main/Exploration ); précédent : 001990; suivant : 001992The Monofunctional Catalase KatE of Xanthomonas axonopodis pv. citri Is Required for Full Virulence in Citrus Plants
Auteurs : María Laura Tondo ; Silvana Petrocelli ; Jorgelina Ottado ; Elena G. OrellanoSource :
- PLoS ONE [ 1932-6203 ] ; 2010.
Abstract
Xac catalase activity was analyzed using native gel electrophoresis and semi-quantitative RT-PCR. We demonstrated that the catalase activity pattern was regulated in different growth stages displaying the highest levels during the stationary phase. KatE was the most active catalase in this phase of growth. At this stage cells were more resistant to hydrogen peroxide as was determined by the analysis of CFU after the exposition to different H2O2 concentrations. In addition, Xac exhibited an adaptive response to hydrogen peroxide, displaying higher levels of catalase activity and H2O2 resistance after treatment with sub-lethal concentrations of the oxidant. In the plant-like medium XVM2 the expression of KatE was strongly induced and in this medium Xac was more resistant to H2O2. A Xac
Our results confirmed that in contrast to other Xanthomonas species, Xac catalase-specific activity is induced during the stationary phase of growth in parallel with the bacterial resistance to peroxide challenge. Moreover, Xac catalases expression pattern is modified in response to any stimuli associated with the plant or the microenvironment it provides. The catalase KatE has been shown to have an important function for the colonization and survival of the bacterium in the citrus plant during the pathogenic process. Our work provides the first genetic evidence to support a monofunctional catalase as a virulence factor in Xac.
Url:
DOI: 10.1371/journal.pone.0010803
PubMed: 20520822
PubMed Central: 2875408
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Monofunctional Catalase KatE of <italic>Xanthomonas axonopodis</italic> pv. <italic>citri</italic> Is Required for Full Virulence in Citrus Plants</title><author><name sortKey="Tondo, Maria Laura" sort="Tondo, Maria Laura" uniqKey="Tondo M" first="María Laura" last="Tondo">María Laura Tondo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Petrocelli, Silvana" sort="Petrocelli, Silvana" uniqKey="Petrocelli S" first="Silvana" last="Petrocelli">Silvana Petrocelli</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Ottado, Jorgelina" sort="Ottado, Jorgelina" uniqKey="Ottado J" first="Jorgelina" last="Ottado">Jorgelina Ottado</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Orellano, Elena G" sort="Orellano, Elena G" uniqKey="Orellano E" first="Elena G." last="Orellano">Elena G. Orellano</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20520822</idno><idno type="pmc">2875408</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875408</idno><idno type="RBID">PMC:2875408</idno><idno type="doi">10.1371/journal.pone.0010803</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">001215</idno><idno type="wicri:Area/Pmc/Curation">001214</idno><idno type="wicri:Area/Pmc/Checkpoint">000F16</idno><idno type="wicri:Area/Ncbi/Merge">000B84</idno><idno type="wicri:Area/Ncbi/Curation">000B84</idno><idno type="wicri:Area/Ncbi/Checkpoint">000B84</idno><idno type="wicri:Area/Main/Merge">001A27</idno><idno type="wicri:Area/Main/Curation">001991</idno><idno type="wicri:Area/Main/Exploration">001991</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Monofunctional Catalase KatE of <italic>Xanthomonas axonopodis</italic> pv. <italic>citri</italic> Is Required for Full Virulence in Citrus Plants</title><author><name sortKey="Tondo, Maria Laura" sort="Tondo, Maria Laura" uniqKey="Tondo M" first="María Laura" last="Tondo">María Laura Tondo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Petrocelli, Silvana" sort="Petrocelli, Silvana" uniqKey="Petrocelli S" first="Silvana" last="Petrocelli">Silvana Petrocelli</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Ottado, Jorgelina" sort="Ottado, Jorgelina" uniqKey="Ottado J" first="Jorgelina" last="Ottado">Jorgelina Ottado</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Orellano, Elena G" sort="Orellano, Elena G" uniqKey="Orellano E" first="Elena G." last="Orellano">Elena G. Orellano</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p><italic>Xanthomonas axonopodis</italic> pv. <italic>citri</italic> (Xac) is an obligate aerobic phytopathogen constantly exposed to hydrogen peroxide produced by normal aerobic respiration and by the plant defense response during plant-pathogen interactions. Four putative catalase genes have been identified <italic>in silico</italic> in the Xac genome, designated as <italic>katE</italic>, <italic>catB</italic>, <italic>srpA</italic> (monofunctional catalases) and <italic>katG</italic> (bifunctional catalase).</p></sec><sec><title>Methodology/Principal Findings</title><p>Xac catalase activity was analyzed using native gel electrophoresis and semi-quantitative RT-PCR. We demonstrated that the catalase activity pattern was regulated in different growth stages displaying the highest levels during the stationary phase. KatE was the most active catalase in this phase of growth. At this stage cells were more resistant to hydrogen peroxide as was determined by the analysis of CFU after the exposition to different H<sub>2</sub>O<sub>2</sub> concentrations. In addition, Xac exhibited an adaptive response to hydrogen peroxide, displaying higher levels of catalase activity and H<sub>2</sub>O<sub>2</sub> resistance after treatment with sub-lethal concentrations of the oxidant. In the plant-like medium XVM2 the expression of KatE was strongly induced and in this medium Xac was more resistant to H<sub>2</sub>O<sub>2</sub>. A Xac<italic>katE</italic> mutant strain was constructed by insertional mutagenesis. We observed that catalase induction in stationary phase was lost meanwhile the adaptive response to peroxide was maintained in this mutant. Finally, the Xac<italic>katE</italic> strain was assayed <italic>in planta</italic> during host plant interaction rendering a less aggressive phenotype with a minor canker formation.</p></sec><sec><title>Conclusions</title><p>Our results confirmed that in contrast to other Xanthomonas species, Xac catalase-specific activity is induced during the stationary phase of growth in parallel with the bacterial resistance to peroxide challenge. Moreover, Xac catalases expression pattern is modified in response to any stimuli associated with the plant or the microenvironment it provides. The catalase KatE has been shown to have an important function for the colonization and survival of the bacterium in the citrus plant during the pathogenic process. Our work provides the first genetic evidence to support a monofunctional catalase as a virulence factor in Xac.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Fridovich, I" uniqKey="Fridovich I">I Fridovich</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Imlay, Ja" uniqKey="Imlay J">JA Imlay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mccord, Jm" uniqKey="Mccord J">JM McCord</name></author><author><name sortKey="Fridovich, I" uniqKey="Fridovich I">I Fridovich</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Green, J" uniqKey="Green J">J Green</name></author><author><name sortKey="Paget, Ms" uniqKey="Paget M">MS Paget</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Imlay, Ja" uniqKey="Imlay J">JA Imlay</name></author><author><name sortKey="Linn, S" uniqKey="Linn S">S 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sortKey="Buranajitpakorn, S" uniqKey="Buranajitpakorn S">S Buranajitpakorn</name></author><author><name sortKey="Vattanaviboon, P" uniqKey="Vattanaviboon P">P Vattanaviboon</name></author><author><name sortKey="Mongkolsuk, S" uniqKey="Mongkolsuk S">S Mongkolsuk</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Orellano, Elena G" sort="Orellano, Elena G" uniqKey="Orellano E" first="Elena G." last="Orellano">Elena G. Orellano</name><name sortKey="Ottado, Jorgelina" sort="Ottado, Jorgelina" uniqKey="Ottado J" first="Jorgelina" last="Ottado">Jorgelina Ottado</name><name sortKey="Petrocelli, Silvana" sort="Petrocelli, Silvana" uniqKey="Petrocelli S" first="Silvana" last="Petrocelli">Silvana Petrocelli</name><name sortKey="Tondo, Maria Laura" sort="Tondo, Maria Laura" uniqKey="Tondo M" first="María Laura" last="Tondo">María Laura Tondo</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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